@article {RICCOBELLI2020103745,
	title = {Surface tension controls the onset of gyrification in brain organoids},
	journal = {Journal of the Mechanics and Physics of Solids},
	volume = {134},
	year = {2020},
	pages = {103745},
	abstract = {<p>Understanding the mechanics of brain embryogenesis can provide insights on pathologies related to brain development, such as lissencephaly, a genetic disease which causes a reduction of the number of cerebral sulci. Recent experiments on brain organoids have confirmed that gyrification, i.e.\&nbsp;the formation of the folded structures of the brain, is triggered by the inhomogeneous growth of the peripheral region. However, the rheology of these cellular aggregates and the mechanics of lissencephaly are still matter of debate. In this work, we develop a mathematical model of brain organoids based on the theory of morpho-elasticity. We describe them as non-linear elastic bodies, composed of a disk surrounded by a growing layer called cortex. The external boundary is subjected to a tissue surface tension due the intercellular adhesion forces. We show that the resulting surface energy is relevant at the small length scales of brain organoids and affects the mechanics of cellular aggregates. We perform a linear stability analysis of the radially symmetric configuration and we study the post-buckling behaviour through finite element simulations. We find that the process of gyrification is triggered by the cortex growth and modulated by the competition between two length scales: the radius of the organoid and the capillary length generated by surface tension. We show that a solid model can reproduce the results of the in-vitro experiments. Furthermore, we prove that the lack of brain sulci in lissencephaly is caused by a reduction of the cell stiffness: the softening of the organoid strengthens the role of surface tension, delaying or even inhibiting the onset of a mechanical instability at the free boundary.</p>},
	keywords = {Buckling, Embryogenesis, Morpho-elasticity, Post-buckling analysis, Surface tension},
	issn = {0022-5096},
	doi = {https://doi.org/10.1016/j.jmps.2019.103745},
	url = {http://www.sciencedirect.com/science/article/pii/S0022509619304065},
	author = {Davide Riccobelli and Giulia Bevilacqua}
}
